2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Mga karaniwang sangkap na ginagamit sa calibration curve ng relatibong distribusyon ng molekular na masa: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrumento at kagamitan
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Sa pangkalahatan, ang proporsyon ng mga amino acid sa mga produkto ng Sustar ay mas mataas kaysa sa mga produkto ng Zinpro.
Bahagi 8 Mga Epekto ng Paggamit
Mga epekto ng iba't ibang pinagmumulan ng mga trace mineral sa pagganap ng produksyon at kalidad ng itlog ng mga inahing manok sa huling panahon ng pangingitlog
Proseso ng Produksyon
Teknolohiya ng naka-target na chelation
Teknolohiya ng shear emulsification
Teknolohiya ng pressure spray at pagpapatuyo
Teknolohiya ng pagpapalamig at dehumidipikasyon
Makabagong teknolohiya sa pagkontrol sa kapaligiran
Apendiks A: Mga Paraan para sa Pagtukoy ng relatibong distribusyon ng molekular na masa ng mga peptide
Pag-aampon ng pamantayan: GB/T 22492-2008
1 Prinsipyo ng Pagsubok:
Ito ay natukoy sa pamamagitan ng high performance gel filtration chromatography. Ibig sabihin, gamit ang porous filler bilang stationary phase, batay sa pagkakaiba sa relatibong laki ng molekular na masa ng mga bahagi ng sample para sa paghihiwalay, na natukoy sa peptide bond ng ultraviolet absorption wavelength na 220 nm, gamit ang nakalaang data processing software para sa pagtukoy ng relatibong distribusyon ng molekular na masa sa pamamagitan ng gel filtration chromatography (ibig sabihin, ang GPC software), ang mga chromatogram at ang kanilang datos ay pinoproseso, kinalkula upang makuha ang laki ng relatibong molekular na masa ng soybean peptide at ang saklaw ng distribusyon.
2. Mga Reagent
Ang tubig na pang-eksperimento ay dapat matugunan ang espesipikasyon ng pangalawang tubig sa GB/T6682, ang paggamit ng mga reagent, maliban sa mga espesyal na probisyon, ay dapat na analitikal na dalisay.
2.1 Kabilang sa mga reagent ang acetonitrile (puro ayon sa chromatographic), trifluoroacetic acid (puro ayon sa chromatographic),
2.2 Mga karaniwang sangkap na ginagamit sa calibration curve ng relatibong distribusyon ng molekular na masa: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrumento at kagamitan
3.1 High Performance Liquid Chromatograph (HPLC): isang chromatographic workstation o integrator na may UV detector at GPC data processing software.
3.2 Yunit ng pagsasala at pag-aalis ng gas gamit ang vacuum para sa mobile phase.
3.3 Elektronikong balanse: gradwadong halaga 0.000 1g.
4 na hakbang sa pagpapatakbo
4.1 Mga kondisyong kromatograpiko at mga eksperimento sa pag-aangkop ng sistema (mga kondisyong sanggunian)
- 4.1.1 Kolum na kromatograpiko: TSKgelG2000swxl300 mm×7.8 mm (panloob na diyametro) o iba pang mga kolum na gel na may parehong uri na may katulad na pagganap na angkop para sa pagtukoy ng mga protina at peptide.
- 4.1.2 Yugto ng paggalaw: Acetonitrile + tubig + trifluoroacetic acid = 20 + 80 + 0.1.
- 4.1.3 Haba ng daluyong ng pagtuklas: 220 nm.
- 4.1.4 Bilis ng daloy: 0.5 mL/min.
- 4.1.5 Oras ng pagtuklas: 30 min.
- 4.1.6 Dami ng iniksyon ng sample: 20μL.
- 4.1.7 Temperatura ng haligi: temperatura ng silid.
- 4.1.8 Upang matugunan ng sistemang chromatographic ang mga kinakailangan sa pagtuklas, itinakda na sa ilalim ng mga kondisyong chromatographic sa itaas, ang kahusayan ng gel chromatographic column, ibig sabihin, ang teoretikal na bilang ng mga plate (N), ay hindi bababa sa 10000 na kinakalkula batay sa mga peak ng pamantayang tripeptide (Glycine-Glycine-Glycine).
- 4.2 Paggawa ng mga karaniwang kurba ng relatibong molekular na masa
- Ang iba't ibang nabanggit na relatibong molekular na masa ng peptide standard solutions na may konsentrasyon ng masa na 1 mg/mL ay inihanda sa pamamagitan ng mobile phase matching, hinalo sa isang tiyak na proporsyon, at pagkatapos ay sinala sa pamamagitan ng organic phase membrane na may pore size na 0.2 μm~0.5 μm at ini-inject sa sample, at pagkatapos ay nakuha ang mga chromatogram ng mga pamantayan. Ang mga relatibong molekular na masa ng pagkakalibrate ng masa at ang kanilang mga equation ay nakuha sa pamamagitan ng pag-plot ng logarithm ng relatibong molekular na masa laban sa oras ng pagpapanatili o sa pamamagitan ng linear regression.
4.3 Paggamot gamit ang sample
Timbangin nang tumpak ang 10mg ng sample sa isang 10mL volumetric flask, magdagdag ng kaunting mobile phase, at i-ultrasonic shaking sa loob ng 10min, upang ang sample ay tuluyang matunaw at maihalo, ma-dilute gamit ang mobile phase hanggang sa timbangan, at pagkatapos ay i-filter sa pamamagitan ng organic phase membrane na may pore size na 0.2μm~0.5μm, at ang filtrate ay sinuri ayon sa mga kondisyon ng chromatographic sa A.4.1.
- 5. Pagkalkula ng relatibong distribusyon ng molekular na masa
- Matapos suriin ang solusyon ng sample na inihanda sa 4.3 sa ilalim ng mga kondisyong chromatographic ng 4.1, ang relatibong molekular na masa ng sample at ang saklaw ng distribusyon nito ay maaaring makuha sa pamamagitan ng pagpapalit ng datos ng chromatographic ng sample sa calibration curve 4.2 gamit ang GPC data processing software. Ang distribusyon ng relatibong molekular na masa ng iba't ibang peptide ay maaaring kalkulahin gamit ang peak area normalization method, ayon sa pormulang: X=A/A total×100
- Sa pormula: X - Ang mass fraction ng isang relatibong molecular mass peptide sa kabuuang peptide sa sample, %;
- A - Pinakamataas na lawak ng isang relatibong molekular na masa ng peptide;
- Kabuuang A - ang kabuuan ng mga peak area ng bawat relatibong molecular mass peptide, na kinalkula sa isang decimal place.
- 6 Pag-uulit
- Ang ganap na pagkakaiba sa pagitan ng dalawang magkakahiwalay na determinasyon na nakuha sa ilalim ng mga kondisyon ng kakayahang maulit ay hindi dapat lumagpas sa 15% ng aritmetikong mean ng dalawang determinasyon.
- Apendiks B: Mga Paraan para sa Pagtukoy ng mga Libreng Amino Acids
- Pag-aampon ng pamantayan: Q/320205 KAVN05-2016
- 1.2 Mga reagent at materyales
- Glacial acetic acid: analitikal na puro
- Asidong perkloriko: 0.0500 mol/L
- Tagapagpahiwatig: 0.1% kristal na lilang tagapagpahiwatig (glacial acetic acid)
- 2. Pagtukoy ng mga libreng amino acid
Ang mga sample ay pinatuyo sa 80°C sa loob ng 1 oras.
Ilagay ang sample sa isang tuyong lalagyan upang natural na lumamig sa temperatura ng silid o palamigin sa magagamit na temperatura.Timbangin ang humigit-kumulang 0.1 g ng sample (tumpak hanggang 0.001 g) sa isang 250 mL na tuyong conical flask.Mabilis na magpatuloy sa susunod na hakbang upang maiwasan ang sample na sumipsip ng ambient moisture.Magdagdag ng 25 mL ng glacial acetic acid at haluing mabuti nang hindi hihigit sa 5 minuto.Magdagdag ng 2 patak ng crystal violet indicatorI-titrate gamit ang 0.0500 mol/L (±0.001) standard titration solution ng perchloric acid hanggang sa magbago ang kulay ng solusyon mula lila patungo sa end point.
Itala ang dami ng karaniwang solusyon na nainom.
- Isagawa ang blankong pagsusulit nang sabay.
- 3. Pagkalkula at mga resulta
- Ang nilalaman ng libreng amino acid na X sa reagent ay ipinapahayag bilang isang mass fraction (%) at kinakalkula ayon sa pormula: X = C × (V1-V0) × 0.1445/M × 100%, sa pormulang ito:
- C - Konsentrasyon ng karaniwang solusyon ng perchloric acid sa moles bawat litro (mol/L)
- V1 - Dami na ginamit para sa titrasyon ng mga sample gamit ang karaniwang solusyon ng perchloric acid, sa mililitro (mL).
- Vo - Dami na ginagamit para sa titration blank gamit ang karaniwang perchloric acid solution, sa milliliters (mL);
M - Masa ng sampol, sa gramo (g).
| 0.1445: Karaniwang masa ng mga amino acid na katumbas ng 1.00 mL ng karaniwang solusyon ng perchloric acid [c (HClO4) = 1.000 mol / L]. | 4.2.3 Pamantayang solusyon ng titrasyon ng Cerium sulfate: konsentrasyon c [Ce(SO4)2] = 0.1 mol/L, inihanda ayon sa GB/T601. | |
| Pag-aampon ng mga pamantayan: Q/70920556 71-2024 | 1. Prinsipyo ng pagtukoy (Fe bilang halimbawa) | Ang mga amino acid iron complex ay may napakababang solubility sa anhydrous ethanol at ang mga free metal ion ay natutunaw sa anhydrous ethanol, ang pagkakaiba sa solubility sa pagitan ng dalawa sa anhydrous ethanol ay ginamit upang matukoy ang chelation rate ng mga amino acid iron complex. |
| Sa pormula: V1 - dami ng karaniwang solusyon ng cerium sulfate na nakonsumo para sa titration ng test solution, mL; | Anhydrous ethanol; ang natitira ay kapareho ng sugnay 4.5.2 sa GB/T 27983-2011. | 3. Mga Hakbang ng Pagsusuri |
| Magsagawa ng dalawang pagsubok nang magkasabay. Timbangin ang 0.1g ng sample na pinatuyo sa 103±2℃ sa loob ng 1 oras, na may katumpakan na 0.0001g, magdagdag ng 100mL ng anhydrous ethanol upang matunaw, salain, at salain ang residue gamit ang 100mL ng anhydrous ethanol nang hindi bababa sa tatlong beses, pagkatapos ay ilipat ang residue sa isang 250mL conical flask, magdagdag ng 10mL ng sulfuric acid solution ayon sa sugnay 4.5.3 sa GB/T27983-2011, at pagkatapos ay isagawa ang mga sumusunod na hakbang ayon sa sugnay 4.5.3 na “Initin upang matunaw at pagkatapos ay hayaang lumamig” sa GB/T27983-2011. Isagawa ang blankong pagsubok nang sabay. | 4. Pagtukoy ng kabuuang nilalaman ng bakal | 4.1 Ang prinsipyo ng pagpapasiya ay kapareho ng sugnay 4.4.1 sa GB/T 21996-2008. |
4.2. Mga Reagent at Solusyon
| 4.2.1 Halo-halong asido: Magdagdag ng 150mL ng sulfuric acid at 150mL ng phosphoric acid sa 700mL ng tubig at haluing mabuti. | 4.2.2 Solusyon ng tagapagpahiwatig ng sodium diphenylamine sulfonate: 5g/L, inihanda ayon sa GB/T603. | 4.2.3 Pamantayang solusyon ng titrasyon ng Cerium sulfate: konsentrasyon c [Ce(SO4)2] = 0.1 mol/L, inihanda ayon sa GB/T601. | |
| 4.3 Mga Hakbang ng Pagsusuri | Magsagawa ng dalawang pagsubok nang magkasabay. Timbangin ang 0.1g ng sample, na may katumpakan na 020001g, ilagay sa isang 250mL na conical flask, magdagdag ng 10mL ng mixed acid, pagkatapos matunaw, magdagdag ng 30ml ng tubig at 4 na patak ng sodium dianiline sulfonate indicator solution, at pagkatapos ay isagawa ang mga sumusunod na hakbang ayon sa sugnay 4.4.2 sa GB/T21996-2008. Isagawa ang blankong pagsubok nang sabay. | 4.4 Representasyon ng mga resulta | Ang kabuuang nilalaman ng bakal na X1 ng mga amino acid iron complex sa mga tuntunin ng mass fraction ng bakal, ang halagang ipinahayag sa %, ay kinalkula ayon sa pormula (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - karaniwang solusyon ng cerium sulfate na ginagamit para sa titration ng blankong solusyon, mL; | V0 - karaniwang solusyon ng cerium sulfate na ginagamit para sa titration ng blankong solusyon, mL; | C - Aktwal na konsentrasyon ng karaniwang solusyon ng cerium sulfate, mol/L5. Pagkalkula ng nilalaman ng bakal sa mga chelateAng nilalamang bakal na X2 sa chelate batay sa mass fraction ng bakal, ang halagang ipinapahayag sa %, ay kinalkula ayon sa pormulang: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Sa pormula: V1 - dami ng karaniwang solusyon ng cerium sulfate na nakonsumo para sa titration ng test solution, mL; | V2 - karaniwang solusyon ng cerium sulfate na ginagamit para sa titration ng blankong solusyon, mL;nom1-Masa ng sample, g. Kunin ang aritmetikong mean ng mga resulta ng parallel determination bilang mga resulta ng determinasyon, at ang ganap na pagkakaiba ng mga resulta ng parallel determination ay hindi hihigit sa 0.3%. | 0.05585 - masa ng ferrous iron na ipinapahayag sa gramo na katumbas ng 1.00 mL ng cerium sulfate standard solution na C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Masa ng sample, g. Kunin ang aritmetikong mean ng mga resulta ng parallel determination bilang mga resulta ng determinasyon, at ang ganap na pagkakaiba ng mga resulta ng parallel determination ay hindi hihigit sa 0.3%. | 6. Pagkalkula ng bilis ng chelationBilis ng Chelation X3, ang halagang ipinapahayag sa %, X3 = X2/X1 × 100Apendiks C: Mga Paraan para sa Pagtukoy ng Chelation Rate ng Zinpro |
Pag-aampon ng pamantayan: Q/320205 KAVNO7-2016
1. Mga reagent at materyales
a) Glacial acetic acid: analitikal na puro; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Pagtukoy ng mga libreng amino acid
2.1 Ang mga sample ay pinatuyo sa 80°C sa loob ng 1 oras.
2.2 Ilagay ang sample sa isang tuyong lalagyan upang natural na lumamig sa temperatura ng silid o palamigin sa magagamit na temperatura.
2.3 Timbangin ang humigit-kumulang 0.1 g ng sample (tumpak hanggang 0.001 g) sa isang 250 mL na tuyong conical flask
2.4 Mabilis na magpatuloy sa susunod na hakbang upang maiwasan ang pagsipsip ng sample ng halumigmig sa paligid.
2.5 Magdagdag ng 25mL ng glacial acetic acid at haluing mabuti nang hindi hihigit sa 5 minuto.
2.6 Magdagdag ng 2 patak ng indicator na kristal na lila.
2.7 Titrate gamit ang 0.0500mol/L (±0.001) standard titration solution ng perchloric acid hanggang sa magbago ang solusyon mula lila patungong berde sa loob ng 15 segundo nang hindi nagbabago ang kulay bilang end point.
2.8 Itala ang dami ng karaniwang solusyon na nainom.
2.9 Isagawa ang blankong pagsusulit nang sabay-sabay.
- 3. Pagkalkula at mga resulta
- Katalan
- Physicochemical parameters
V1 - Dami na ginamit para sa titrasyon ng mga sample gamit ang karaniwang solusyon ng perchloric acid, sa mililitro (mL).
Vo - Dami na ginagamit para sa titration blank gamit ang karaniwang perchloric acid solution, sa milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Tirahan: No.147 Qingpu Road, Shouan Town, Pujiang County, Chengdu City, Sichuan Province, China
Telepono: 86-18880477902
Mga Produkto
Mga mineral na hindi organiko
- Mga organikong bakas na mineral
- Swahili
- Pasadyang serbisyo
- Mga mabilisang link
Profile ng Kumpanya
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Mag-click para sa pagtatanong | © Karapatang-ari - 2010-2025: Lahat ng Karapatan ay Nakalaan. | Mapa ng Site PINAKAMAHUSAY NA PAGHAHANAP Telepono |
| Telepono | 86-18880477902 | Javanese | E-mail |
| 8618880477902 | Tsino | Pranses | |
| Bird | Tsino | Pranses | Aleman Espanyol |
| Aquatic animals | Hapon | Koreano | Arabe Griyego |
| Turko | Italyano | ||
| Ruminant animal g/head day | January 0.75 | Indones Afrikaans Suweko |
Polish
- Basque
- Katalan
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgaro
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroato
Olandes
| Application object | Urdu Vietnamese | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haitian | Hausa | Kinyarwanda Hmong Hungarian |
| Piglets and fattening pigs | Igbo | Javanese | Kannada Khmer Kurd |
| Kirghiz | Latino | ||
| Bird | 300～400 | 45～60 | Macedonian Malayo Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norwegian
- Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbyo
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili
Tajik
Tamil
Telugu
Thai
| Application object | Urdu Vietnamese | Content in full-value feed (mg/kg) | Efficacy |
| Yiddish | Yoruba | Zulu | Kinyarwanda Oriya Mga Turkmen |
| Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
